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Product Infomation
Product Name Recombinant Human Neurotrophic Factor 3, His
Catalog Number NGF-002
Size 10μg;100μg;1mg
Description Neurotrophin-3 (NT-3) is a neurotrophin belonging to the nerve growth factor (NGF) family and plays a crucial role in the survival of newly formed neurons. NT-3 was discovered by Maisonpierre et al. in 1990 using PCR amplification and cloning techniques. It consists of 119 amino acid residues, has a molecular weight of 13.6 kDa, and is also known as hippocampal neurotrophin. This protein consists of 257 amino acids, with a 138-amino-acid pro-region and a mature sequence of 119 residues. Human NT-3 has been used for magnetic sorting of oligodendrocyte progenitor cells. NT-3 (13.6 kDa or 27.2 kDa dimer) is a member of the neurotrophin family. It shares the same amino acid sequence in humans, mice, and pigs and exhibits complete cross-reactivity. NT-3 is important for the development and maintenance of neuronal populations and promotes the differentiation of neural crest-derived sensory and sympathetic neurons. This is crucial for type I afferent neurons of the somatosensory system, which transmit information from peripheral muscle spindles to motor neurons and project to spinocerebellar neurons. NT-3 is also important in the upper cervical and nodal ganglia.
Application This product can be used for research on supporting neuronal survival and differentiation in peripheral nerve regeneration using His-tagged human neurotrophic factor 3.
Molecular Weight 15 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity Its activity was evaluated by detecting its growth inhibition ability on Mv.1.Lu lung epithelial cells. ED₅₀ < 0.1 ng/mL, biological activity > 1×10⁷ IU/mg.
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
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