Fluorescent microspheres are a class of functional microspheres containing fluorescent materials that emit fluorescence when exposed to specific excitation light. Matexcel is committed to providing fluorescent microspheres with uniform particle size, high fluorescence intensity, and excellent photostability for applications in biopharmaceuticals, in vitro diagnostics, cell tracking, flow cytometry, and environmental monitoring. Our microspheres can be customized with various surface functional groups and emission wavelengths to meet client needs, from research and development to industrial-scale production.

Method for Preparing Fluorescent Microspheres

a) Embed fluorescent dyes or fluorescent nanoparticles within or on the surface of the microspheres.

b) Perform solvent exchange on porous microspheres to cause them to shrink and immobilize the fluorescent particles.

c) Ensure that the microspheres are monodisperse, have uniform particle sizes, and possess stable physicochemical properties.

Applications of Fluorescent Microspheres

  • In Vitro Diagnostics and Biomedical Research: Used as internal standards or tracers for lateral flow (immunoassay), chemiluminescence, and PCR amplification, and for kit development and the visual assessment of drug delivery systems.
  • Life Sciences Research: Supports instrument calibration and compensation for flow cytometry, as well as studies of cell phagocytosis, endocytosis mechanisms, and multicolor cell tracking under confocal microscopy.
  • Medical Device Quality Control: Serves as a reference standard for linear calibration, sensitivity validation, and daily quality control of hematology analyzers and urinalysis analyzers.
  • Environmental and Materials Science: Tracks the migration of microplastics and heavy metal nanoparticles in plant or environmental systems.

Types of Fluorescence

Fluorescence Type Best Excitation Wavelength (nm) Best Emission Wavelength (nm) Typical Dyes/Technologies Applications
Blue 365 415-460 Standard blue dyes, AIE blue light Routine fluorescent labeling, flow cytometry, cell tracing.
Green 400-500 515-530 FITC, AIE green light Immunochromatography, bioimaging, flow cytometry.
Yellow/Orange-Red 540-560 560-605 Standard yellow/orange dyes Multicolor labeling, confocal microscope calibration.
Red 365 (UV) or 540-580 (visible light) 610-640 Rare earth europium chelates, AIE red light High-sensitivity immunoassay, time-resolved fluorescence analysis.
Deep Red/Near-Infrared 660-725 680-750 Near-infrared dyes In vivo imaging, tissue penetration detection, multicolor analysis.

If you require microspheres with specific wavelength combinations or surface functional groups (such as carboxyl groups or streptavidin), please contact us for a more detailed customization proposal.

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