We can offer a discount on bulk orders. Please contact our sales team for further inquiry.
Product Infomation
Product Name Recombinant Human Nerve Growth Factor-β, His
Catalog Number NGF-001
Size 10μg;100μg;1mg
Description NGF consists of 2 α, 1 β, and 2 γ subunits, with the chemical formula α2βγ2 and a molecular weight of approximately 130,000. The α, β, and γ subunits possess trypsin-like activity, an insulin-like structure, and a serine protease structure, respectively. Among these, the β subunit is the active site that promotes nerve growth; it is a dimer formed by a single chain of 118 amino acid residues linked via non-covalent bonds. β-NGF is a potent neurotrophic factor that signals through its receptor, β-NGFR, and plays a crucial role in the development and maintenance of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, enhancing B-cell survival. NGF also possesses osteogenic activity. The functional form of recombinant human β-NGF is a non-covalently linked homodimer composed of two 14 kDa polypeptide subunits, each containing 120 amino acids and three disulfide bonds.
Application This product can be used for research on inducing neuronal survival and axonal outgrowth in neural tissue engineering using human NGF-β with His tag for purification.
Molecular Weight 14 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity Determined by the method promoting the proliferation of Balb/c 3T3 cells. ED₅₀ < 1 ng/mL, biological activity > 1×10⁶ IU/mg.
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
Online Inquiry
For Research or Industrial Raw Materials, Not For Personal Medical Use!
Place the Order
FAQs
logo

Enter your email here to subscribe.

Copyright © MATEXCEL. All Rights Reserved.
0
Inquiry Basket