We can offer a discount on bulk orders. Please contact our sales team for further inquiry.
Product Infomation
Product Name Recombinant Human Brain-Derived Neurotrophic Factor, His
Catalog Number NGF-003
Size 10μg;100μg;1mg
Description Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophic factor family, primarily expressed in the hippocampus, amygdala, cerebral cortex, hypothalamus, and cerebellum, and is also present in platelets and circulating plasma. BDNF is a protein with a molecular weight of 27.8 kDa, containing 247 amino acid residues, and plays a key role in regulating synaptic transmission and plasticity in various regions of the central nervous system. Additionally, BDNF acts as a regulator of long-term potentiation, a memory-related modification in hippocampal synaptic transmission. The precursor form of BDNF preferentially interacts with the pan-neurotrophic receptor p75 (P75NTR) and the VPS10P-domain-containing receptor sorbitol, inducing neuronal apoptosis and playing a significant role in the negative regulation of neurotrophic effects in the brain. Mature BDNF selectively binds to the TrkB kinase receptor, promoting neuronal survival, growth, and differentiation.
Application This product can be used for research on promoting neurogenesis and synaptic plasticity in neural tissue engineering with His-tagged human BDNF for enhanced stability.
Molecular Weight 13.5 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
Online Inquiry
For Research or Industrial Raw Materials, Not For Personal Medical Use!
Place the Order
FAQs
logo

Enter your email here to subscribe.

Copyright © MATEXCEL. All Rights Reserved.
0
Inquiry Basket