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Product Infomation
Product Name Recombinant Mouse Keratinocyte Growth Factor-2
Catalog Number KGF-003
Size 5µg;25µg;1mg
Description KGF-2 is a member of the fibroblast growth factor (FGF) family. Members of the FGF family possess broad mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF-10 exhibits mitogenic activity toward keratinized epidermal cells but has almost no activity toward fibroblasts, a pattern similar to the biological activity of FGF-7. Studies of the mouse homologous gene indicate that this gene is essential for embryonic epidermal morphogenesis, including brain development, lung morphogenesis, and the initiation of limb bud formation. The gene is also believed to play a critical role in the wound healing process.
Application This product can be used for research on supporting murine epithelial regeneration with mouse KGF-2.
Molecular Weight 19.5 kDa
Species Mouse
Source E.coli
Appearance Sterile-filtered white lyophilized powder
Purity ≥ 97% by SDS-PAGE, RP-HPLC
Activity It exhibits full biological activity compared to the standard. As determined by the thymidine uptake assay in BaF3 cells expressing FGF receptors following dose-dependent stimulation, the ED₅₀ is < 0.5 ng/mL.
Redissolution It is recommended to resuspend the lyophilized KGF-2 recombinant mouse protein in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/mL, after which it can be further diluted into other aqueous solutions.
Formulation Freeze-dry the concentrated solution (1×PBS, pH 7.4, containing 5% trehalose) after filtering it through a 0.2 µm filter.
Amino Acid Sequence QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT
Storage Store at room temperature for 3 weeks, or in a dry environment at -18°C or below. After reconstitution, store at 4°C for 2-7 days; thereafter, store at -18°C or below for future use. Avoid repeated freeze-thaw cycles.
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