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Product Infomation
Product Name Recombinant Mouse Acidic Fibroblast Growth Factor
Catalog Number FGF-010
Size 10µg;50µg;1mg
Description Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. Members of the FGF family possess broad mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This protein acts as a regulator of endothelial cell migration and proliferation and is also a angiogenic factor. In vitro, it acts as a mitogen for various mesodermal and neuroectodermal cells and is therefore believed to be involved in organogenesis. Currently, three selectively spliced variants encoding different subtypes have been identified. Bound growth factors exhibit angiogenic effects in vivo and possess potent mitogenic activity on various cell types in vitro. These two growth factors differ in their tissue distribution and concentration.
Application This product can be used for research on facilitating murine-specific cell culture models for tissue engineering research with mouse acidic FGF.
Molecular Weight 15.8 kDa
Species Mouse
Source E.coli
Appearance Sterile-filtered white lyophilized powder
Purity ≥ 96% by SDS-PAGE, RP-HPLC
Activity The ED₅₀ determined by a dose-dependent proliferation assay using mouse BALB/c 3T3 cells was less than 0.2 ng/mL, corresponding to a specific activity of 5 × 10⁶ IU/mg.
Redissolution It is recommended to resuspend the lyophilized acidic fibroblast growth factor in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/mL, after which it can be further diluted into other aqueous solutions.
Formulation Lyophilize the concentrated solution (PBS, pH 7.0, 5% trehalose, 0.02% Tween-80, 0.5 mM DTT, and 0.5 mM EDTA) after filtering through a 0.2 µm filter.
Amino Acid Sequence MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Storage Store at room temperature for 3 weeks, or in a dry environment at -18°C or below. After reconstitution, store at 4°C for 2-7 days; thereafter, store at -18°C or below for future use. Avoid repeated freeze-thaw cycles.
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