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Product Infomation
Product Name Recombinant Human Vascular Endothelial Growth Factor E
Catalog Number VGF-007
Size 2µg;10µg;1mg
Description Based on sequence similarity to VEGF-A, a gene encoding a VEGF homolog was recently identified in the genome of the sheep oral herpes virus (Orf virus, OV) (Lyttle et al., 1994). Different strains of the sheep oral vesicular disease virus exhibit significant amino acid sequence similarity to VEGF-A and have been described as a viral virulence factor that appears to have originated from a captured host gene. All eight cysteine residues in the central cysteine motif, which is characteristic of VEGF family members, are as conserved in the VEGF-E protein as they are in other residues. Alignment of all mammalian VEGF sequences indicates that VEGF-E differs from previously described VEGFs but is most closely related to VEGF-A. Similar to VEGF-A, VEGF-E has been found to bind to VEGF receptor 2 (KDR) with high affinity, leading to receptor autophosphorylation. However, unlike VEGF-A, VEGF-E cannot bind to VEGF receptor 1 (Flt-1). Furthermore, VEGF-E cannot bind to VEGF receptor 3 (FLT-4) either. Therefore, VEGF-E is a potent angiogenic factor.
Application This product can be used for research on inducing vascular permeability and angiogenesis with viral homolog human VEGF-E.
Molecular Weight 35 kDa
Species Human
Source E.coli
Appearance Sterile-filtered white lyophilized powder
Purity ≥ 90% by SDS-PAGE
Activity Bioactivity assays include: (1) testing its ability to induce VEGFR-2/KDR receptor phosphorylation in PAE/KDR cells; (2) testing its effect in primary HUVEC cell proliferation assays. The ED₅₀ for this effect is typically 1-5 ng/mL.
Redissolution Lyophilized oVEGF-E Orf virus should be resuspended in water or culture medium to a concentration of at least 50 µg/mL. For long-term storage, it is recommended to add at least 0.1% human serum or bovine serum albumin.
Formulation This protein was lyophilized from a concentrated (1 mg/mL) solution containing PBS.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH DSTKTWSEVF ENSGCKPRPM VFRVHDEHPE LTSQRFNPPC VTLMRCGGCC NDESLECVPT EEANVTMQLM GASVSGGNGM QHLSFVEHKK CDCKPPLTTT PPTTTRPPRR RR
Storage Store at room temperature for 3 weeks, or in a dry environment at -18°C or below. After reconstitution, store at 4°C for 2-7 days; thereafter, store at -18°C or below for future use. Avoid repeated freeze-thaw cycles.
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