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Product Infomation
Product Name Recombinant Human Stem Cell Growth Factor-α, His
Catalog Number OGF-002
Size 10μg;100μg;1mg
Description Stem Cell Growth Factor (SCGF) is a non-glycosylated, species-specific cytokine belonging to the C-type lectin superfamily, with two subtypes: α and β. SCGF is a hematopoietic growth factor that exerts its biological activity during the early stages of hematopoiesis, primarily supporting the growth of primitive hematopoietic cells. When used in combination with EPO or S-CGF, it can promote proliferation. SCGF is highly expressed in bone marrow and possesses osteogenic activity, promoting bone growth. This product is human SCGF-α (YKYKY), 321 amino acids, 34.9 kDa, containing His and YKYKY. The latter can be converted into a DOPA structure under the action of tyrosinase and exhibits broad adhesion to matrix materials.
Application This product can be used for research on maintaining pluripotency and self-renewal of stem cells in culture systems with His-tagged human stem cell growth factor-α.
Molecular Weight 34.9 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity The osteogenic differentiation effect was evaluated by detecting the ALP level of BMSCs, with an effective concentration of 1-10 pM (0.035-0.35 ng/mL).
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
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