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Product Infomation
Product Name Recombinant Human Receptor Activator of Nuclear Factor κB Ligand, His
Catalog Number OGF-005
Size 10μg;100μg;1mg
Description RANKL and RANK are members of the TNF superfamily of ligands and receptors, playing important roles in regulating specific immunity and bone remodeling. RANKL is expressed in various cell types, including osteoblasts, fibroblasts, activated T cells, and bone marrow stromal cells, and it is capable of interacting with a decoy receptor known as OPG. The binding of soluble OPG to sRANKL inhibits osteoclastogenesis by blocking signal transduction between stromal cells and osteoclast precursor cells, thereby preventing excessive accumulation of bone and cartilage. Human RANKL is active against mouse cells. Recombinant human sRANKL is a 20.0 kDa polypeptide consisting of the TNF-homologous region of RANKL (176 amino acid residues).
Application This product can be used for research on inducing osteoclastogenesis for bone remodeling studies in tissue engineering using His-tagged human RANKL for precise signaling.
Molecular Weight 20 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity Determined by its ability to induce acid phosphatase (ACP) production in RAW264.7 cells. ED₅₀ < 50 ng/mL.
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
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