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Product Infomation
Product Name Recombinant Human Insulin-like Growth Factor-1, His
Catalog Number OGF-001
Size 10μg;100μg;1mg
Description The IGF family is a class of mitogenic growth factors that can stimulate the proliferation of various cell types, including myocytes, osteocytes, and chondrocytes, in vitro. IGF is primarily produced by the liver and is structurally and functionally similar to insulin, but possesses significantly higher growth-promoting activity than insulin. Mature IGF is produced by the hydrolysis of an inactive precursor protein and contains N-terminal and C-terminal propeptide regions.
Application This product can be used for research on mediating anabolic effects on cartilage and bone in engineered grafts with His-tagged human IGF-1 for enhanced bioavailability.
Molecular Weight 8.5 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity Determined by the method promoting the proliferation of Balb/c 3T3 cells. ED₅₀ < 1 ng/mL, biological activity > 1×10⁶ IU/mg.
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
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