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Product Infomation
Product Name Recombinant Human Hepatocyte Growth Factor, HEK
Catalog Number HGF-004
Size 2µg;10µg;1mg
Description Hepatocyte Growth Factor (HGF) is a multifunctional growth factor that regulates cell growth and cell migration. It exerts a potent mitogenic effect on hepatocytes and primary epithelial cells. HGF acts synergistically with Interleukin-3 and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) to stimulate colony formation in hematopoietic progenitor cells in vitro, and may therefore also regulate the hematopoietic process.
Application This product can be used for research on facilitating human-specific HGF studies in tissue engineering with HEK-derived human HGF.
Molecular Weight 80 kDa
Species Human
Source HEK
Appearance Sterile-filtered white lyophilized powder
Purity ≥ 90% by SDS-PAGE
Activity This activity was determined using cell proliferation assays with 4MBr-5 rhesus monkey epithelial cells. The EC₅₀ for this effect is typically 20-40 ng/mL.
Redissolution It is recommended to reconstitute the lyophilized hepatocyte growth factor with sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/mL, after which it may be further diluted into other aqueous solutions.
Formulation HGF was lyophilized from a solution (1 mg/mL) containing 10 mM sodium phosphate, 150 mM NaCl, 0.01% Tween 80, and 100 mM L-arginine, pH 6.5.
Amino Acid Sequence 32-QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRVVNGIPT RTNIGWMVSL RYRNKHICGG SLIKESWVLT ARQCFPSRDL KDYEAWLGIH DVHGRGDEKC KQVLNVSQLV YGPEGSDLVL MKLARPAVLD DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRV AHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSGPCEG DYGGPLVCEQ HKMRMVLGVI VPGRGCAIPN RPGIFVRVAY YAKWIHKIIL TYKVPQS-728
Storage Store at room temperature for 3 weeks, or in a dry environment at -18°C or below. After reconstitution, store at 4°C for 2-7 days; thereafter, store at -18°C or below for future use. Avoid repeated freeze-thaw cycles.
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