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Product Infomation
Product Name Recombinant Human Fibronectin, His
Catalog Number OGF-007
Size 10μg;100μg;1mg
Description Fibronectin is a large extracellular membrane protein present on the cell surface and is the primary non-collagenous glycoprotein in the extracellular matrix and basement membrane. Fn plays a central role in cell adhesion; it recognizes and binds to integrin heterodimers via the arginine-glycine-aspartic acid (Arg-Gly-Asp, RGD) sequence on fibronectin III10, thereby promoting intercellular adhesion and cell-matrix adhesion. Fn also promotes cell migration, growth, and differentiation; under the influence of Fn, cells spread rapidly and maintain good cellular morphology. Fn promotes fibroblast proliferation, induces epidermal cells to migrate through granulation tissue, and facilitates the reconstruction of the subepidermal basement membrane and normal keratinization processes. The sequence of this product primarily includes two fragments of natural fibronectin, FnIII9 and FnIII10, and contains a purification tag.
Application This product can be used for research on enhancing cell adhesion and migration on biomaterial surfaces for tissue integration using His-tagged human fibronectin for surface coating.
Molecular Weight 20 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity Cultured with semi-adherent colon cancer HCT116 cells for 1-5 days. The effective concentration for promoting adhesion and proliferation is 1.25-2.5 μg/mL.
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
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