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Product Infomation
Product Name Recombinant Human Epidermal Growth Factor Long
Catalog Number EGF-008
Size 20µg;100µg;1mg
Description Epidermal growth factor (EGF) has a significant influence on the differentiation of specific cells in vivo and is a potent mitogen for cultured cells of various ectodermal and mesodermal origins. The EGF precursor is believed to be a membrane-bound molecule that, upon cleavage by proteases, yields a 53-amino acid peptide hormone capable of stimulating cell division. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro, as well as the growth of certain fibroblasts in cell culture. Long-chain EGF is a recombinant analog of human EGF, developed to serve as a substitute for recombinant human or native EGF in therapeutic cell culture applications. It comprises the amino acid sequence of human EGF along with a 53-amino acid N-terminal extension peptide.
Application This product can be used for research on extending EGF half-life and bioactivity in long-term tissue engineering cultures with human EGF long isoform.
Molecular Weight 12.3 kDa
Species Human
Source E.coli
Appearance Sterile-filtered white lyophilized powder
Purity ≥ 95% by SDS-PAGE, RP-HPLC
Activity The ED₅₀ determined by a cell proliferation assay using mouse Balb/c 3T3 cells is less than 1.0 ng/mL, equivalent to an activity of > 1.0 ×10⁶ IU/mg.
Redissolution It is recommended to reconstitute lyophilized EGF Long with sterile 100 mM acetic acid (AcOH) solution to a concentration of at least 100 µg/mL, after which it may be further diluted into other aqueous solutions.
Formulation EGF Long was lyophilized from a concentrated solution filtered through a 0.2 µm filter from 10 mM HCl.
Amino Acid Sequence MFPAMPLSSL FANAVLRAQH LHQLAADTYK EFERAYIPEG QRYSIQVNFA HYGNSDSECP LSHDGYCLHD GVCMYIEALD KYACNCVVGY IGERCQYRDL KWWELR
Storage Store at room temperature for 3 weeks, or in a dry environment at -18°C or below. After reconstitution, store at 4°C for 2-7 days; thereafter, store at -18°C or below for future use. Avoid repeated freeze-thaw cycles.
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