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Product Infomation
Product Name Recombinant Human Epidermal Growth Factor
Catalog Number EGF-003
Size 10μg;100μg;1mg
Description Epidermal growth factor (EGF) is a growth factor belonging to a protein family whose extracellular domain contains at least one domain composed of six conserved cysteines forming three disulfide bonds (EGF structural unit). EGF promotes the proliferation and differentiation of mesenchymal and epithelial cells. In vitro, EGF acts as a mitogen for fibroblasts, epithelial cells, and endothelial cells, promoting colony formation in epithelial cell cultures. In vivo, EGF promotes the formation of epithelial cells and blood vessels and inhibits gastric acid secretion.
Application This product can be used for research on supporting maintenance and expansion of stem cells in tissue-engineered constructs using human EGF without affinity tags.
Molecular Weight 6.0 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity Determined by the method promoting the proliferation of Balb/c 3T3 cells. ED₅₀ < 1 ng/mL, biological activity > 1×10⁶ IU/mg.
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
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