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Product Infomation
Product Name Recombinant Human Epidermal Growth Factor, 1-51 a.a.
Catalog Number EGF-006
Size 100µg;0.5mg;1mg
Description Epidermal growth factor (EGF) has a significant influence on the differentiation of specific cells in vivo and is a potent mitogen for cultured cells of various ectodermal and mesodermal origins. The EGF precursor is considered a membrane-bound molecule that, upon cleavage by proteases, yields a 53-amino acid peptide hormone capable of stimulating cell division. EGF stimulates the growth of various epidermal and epithelial tissues both in vivo and in vitro, and promotes the growth of certain fibroblasts in cell culture.
Application This product can be used for research on providing a truncated EGF variant (1-51 a.a.) for specific receptor activation studies in tissue engineering.
Molecular Weight 6.0 kDa
Species Human
Source Saccharomyces cerevisiae
Appearance Sterile-filtered white lyophilized powder
Purity ≥ 98% by SDS-PAGE, RP-HPLC
Activity The ED₅₀ was determined using a cell proliferation assay with Balb/c 3T3 mouse cells; the value was less than 0.1 ng/mL, corresponding to an activity greater than 1.0 × 10⁷ IU/mg.
Redissolution It is recommended to reconstitute the lyophilized epidermal growth factor in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/mL, after which it may be further diluted into other aqueous solutions.
Formulation Lyophilized from a concentrated PBS solution filtered through a 0.2 µm filter, pH 7.4.
Amino Acid Sequence NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW E
Storage Store at room temperature for 3 weeks, or in a dry environment at -18°C or below. After reconstitution, store at 4°C for 2-7 days; thereafter, store at -18°C or below for future use. Avoid repeated freeze-thaw cycles.
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