We can offer a discount on bulk orders. Please contact our sales team for further inquiry.
Product Infomation
Product Name Recombinant Human Chitin-Binding Basic Fibroblast Growth Factor, Chitin-Specific Binding, His
Catalog Number FGF-004
Size 10μg;100μg;1mg
Description Basic fibroblast growth factor (bFGF) is a multifunctional cell growth factor that acts extensively on tissues and cells of mesodermal and neuroectodermal origin. Its primary biological function is to promote cell division and proliferation. Another important function of bFGF is its angiogenic activity. It can rapidly induce the formation of new blood vessels in tissues such as the cornea, skin, and renal capsule; it chemotaxes various cells of the vascular endothelium and stimulates the proliferation and migration of various cell types, thereby playing a significant role in processes such as tumorigenesis, the repair of tissue damage caused by atherosclerotic plaques, and other related processes. bFGF also acts as a neurotrophic factor, promoting the growth, differentiation, and survival of neural tissues. bFGF plays a crucial role in inducing embryonic development. Normal embryonic development relies heavily on mesodermal induction; bFGF acts on various mesoderm-derived cells to participate in this process. Numerous experiments have confirmed that bFGF can inhibit apoptosis. Chitin, also known as chitin, is the only positively charged animal polysaccharide found in nature. Chitosan is deacetylated chitin, typically with a degree of deacetylation exceeding 85%. Deacetylated chitosan possesses a certain degree of solubility and is typically dissolved using dilute acid solutions or by heating. Due to its positive charge, chitosan exhibits good cell affinity. ChiBD-bFGF binds strongly to chitin or chitosan, and modified chitosan membranes or microcarriers demonstrate significantly enhanced biological activity.　
Application This product can be used for research on enabling targeted growth factor delivery via chitin-based biomaterials in tissue engineering using chitin-binding FGF with His tag.
Feature ChiBD-bFGF contains specific domains that bind to chitin or chitosan, enabling it to recognize and stably bind to these materials, thereby exerting a long-lasting effect.
Molecular Weight 23.6 kDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity Assayed by the method promoting the proliferation of Balb/c 3T3 cells. ED₅₀ < 0.1 ng/mL, biological activity > 1×10⁷ IU/mg, superior to conventional bFGF.
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
Online Inquiry
For Research or Industrial Raw Materials, Not For Personal Medical Use!
Place the Order
FAQs
logo

Enter your email here to subscribe.

Copyright © MATEXCEL. All Rights Reserved.
0
Inquiry Basket