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Product Infomation
Product Name Recombinant Bovine Transforming Growth Factor-β, His
Catalog Number TGF-001
Size 10μg;100μg;1mg
Description Transforming growth factor-β (TGF-β) belongs to the recently discovered TGF-β superfamily, which regulates cell growth and differentiation. TGF-β is a dimer composed of two structurally identical or similar subunits with a molecular weight of 12.5 kDa, linked by disulfide bonds. Initial research on the biological functions of TGF-β focused primarily on inflammation, tissue repair, and embryonic development. In recent years, it has been discovered that TGF-β plays a significant regulatory role in cell growth, differentiation, and immune function. TGF-β1, β2, and β3 have similar functions; generally, TGF-β stimulates cells of mesenchymal origin while inhibiting cells of epithelial or neuroectodermal origin.
Application This product can be used for research on regulating extracellular matrix deposition and cell differentiation in tissue repair with His-tagged bovine TGF-β for species-specific studies.
Molecular Weight 15 kDa
Species Bovine
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Activity Its activity was evaluated by detecting its growth inhibition ability on Mv.1.Lu lung epithelial cells. ED₅₀ < 0.1 ng/mL, biological activity > 1×10⁷ IU/mg.
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
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