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Product Infomation
Product Name Recombinant Bovine Acidic Fibroblast Growth Factor
Catalog Number FGF-008
Size 1µg;5µg;100µg
Description Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. Members of the FGF family possess broad mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This protein acts as a regulator of endothelial cell migration and proliferation and is also a angiogenic factor. In vitro, it acts as a mitogen for various mesodermal and neuroectodermal cells and is therefore believed to be involved in organogenesis. Currently, three selectively spliced variants encoding different subtypes have been identified. Bound growth factors exhibit angiogenic effects in vivo and possess potent mitogenic activity on various cell types in vitro. These two growth factors differ in their tissue distribution and concentration.
Application This product can be used for research on supporting chondrogenesis and osteogenesis in musculoskeletal tissue engineering with bovine acidic FGF.
Molecular Weight 37 kDa
Species Bovine Brain
Source E.coli
Appearance Sterile-filtered white freeze-dried powder
Purity > 90%
Activity Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25 ng/mL FGF.
Redissolution It is recommended to resuspend the lyophilized aFGF in a sterile solution of 50 mM Na₂HPO₄ (pH 7) and 0.5% albumin. The recommended concentration for cell culture is 1-20 ng/mL.
Formulation Lyophilize 5 µg of aFGF from a 0.5 mL solution containing 1 mM sodium phosphate, pH 7, and then filter through a low-binding membrane.
Storage Store at room temperature for 2 weeks, or in a dry environment at -18°C or below. After reconstitution, store at 4°C for 2-3 weeks; thereafter, store at -18°C or below for future use. Avoid repeated freeze-thaw cycles.
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