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Product Infomation
Product Name NHS Magnetic Beads, 2 μm
Catalog Number MGB-IP-029
Size 50 mL
Physical Specs 2 μm
Description NHS-activated magnetic beads feature surface NHS groups that form stable amide linkages with primary amine-containing proteins and molecules, facilitating affinity purification of antibodies, antigens, and other biomolecules. No pre-activation with EDC/NHS or glutaraldehyde is needed. Ligands are dissolved in the supplied coupling buffer and incubated with beads for 1-2 hours at room temperature for rapid, mild, and efficient conjugation. All procedures require amine-free buffers; magnetic separation is performed manually with a magnetic rack or automated for high-throughput applications.
Applications Suitable for covalent coupling of protein, antibody, enzyme, polypeptide, nucleic acid and other biological molecules containing primary amine groups.
Features No activation is required; direct covalent conjugation with biomolecular ligands is possible; highly efficient, with biomolecular ligand conjugation yields exceeding 90%, significantly higher than those of carboxyl-functionalized magnetic beads, while also offering higher ligand loading capacity; biomolecular ligand conjugation can be completed in 1-2 hours; mild conditions—conjugation at room temperature or 4°C with a conjugation system pH of 5-9; forms stable amide bonds to prevent ligand detachment; Excellent biocompatibility, reducing nonspecific adsorption.
Particle Size 2 μm
Concentration 10 mg/mL
Shell Polymer
Storage Solution DMAC
Ligand-binding Capacity ≥ 30 μg IgG/mg beads
Storage 2-8°C
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