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| Product Name | NHS Agarose Magnetic Beads, 10-30 µm |
| Catalog Number | MGB-PP-025 |
| Size | 50 mL, 1000 mL |
| Physical Specs | 10-30 μm |
| Description | This product comprises porous hydrophilic agarose microspheres (10-30 μm) surface-modified with NHS groups, forming stable amide bonds with primary amine-containing proteins for affinity purification or detection. No pre-activation with EDC/NHS or glutaraldehyde is needed; ligands are dissolved in coupling buffer and incubated for 1-2 hours at room temperature for efficient conjugation. |
| Applications | Covalent coupling of biomolecules containing primary amines, such as proteins, antibodies, enzymes, peptides, and nucleic acids. |
| Features | No activation is required; direct covalent coupling with biological ligands is possible; biological ligand coupling efficiency can reach over 90%, significantly higher than that of carboxyl-functionalized magnetic beads, while also offering higher ligand loading capacity; Bioligand conjugation can be completed in 1-2 hours; the process is mild, performed at room temperature or 4°C, with a conjugation system pH of 5-9; stable amide bonds are formed to prevent ligand detachment; excellent biocompatibility reduces nonspecific adsorption. |
| Particle Size | 10-30 μm |
| Concentration | 20% (v/v) |
| Shell | Agarose |
| Storage Solution | Anhydrous isopropanol |
| Ligand-binding Capacity | 20-30 mg IgG/mL beads |
| Storage | 2-8°C |
| Shelf Life | 1 year |