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Product Infomation
Product Name NHS-Activated Agarose Magnetic Beads, 20-45 μm
Catalog Number MGB-BM-027
Physical Specs 20-45 μm
Description NHS-activated agarose magnetic beads (Magarose-NHS) allow straightforward and efficient covalent immobilization of proteins onto magnetic bead supports, offering a reliable method for affinity purification of antibodies, antigens, and other biomolecules. The activated agarose beads contain N-hydroxysuccinimide (NHS) functional groups that react with primary amines on proteins or other molecules to form stable amide bonds. Coupling reactions are performed in amine-free buffers at pH 7-9, with efficiency exceeding 80% independent of ligand molecular weight or isoelectric point (pI). Following ligand immobilization, the resulting agarose magnetic beads can be applied in multiple affinity purification workflows.
Applications Affinity purification of antibodies, antigens or other biological molecules
Particle Size 20-45 μm
Concentration 25% (v/v)
Surface Groups ~50 μmol NHS/mL beads
Shell 6% cross-linked magnetic agarose
Storage Solution 100% isopropyl alcohol
Ligand-binding Capacity >20 mg IgG/mL beads
Storage 2-8°C
Shelf Life 1 year
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