Anti-V5 Magnetic Beads

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Product Infomation

Product Name	Anti-V5 Magnetic Beads
Catalog Number	MGB-IP-021
Size	0.5 mL×1, 0.5 mL×4
Physical Specs	200 nm
Description	Anti-V5 magnetic beads are produced by covalently coupling high-quality mouse monoclonal anti-V5 antibody to nanoscale magnetic beads, which specifically recognize V5-tagged proteins in plant, animal or microbial lysates, serum, ascites and other specimens for IP, Co-IP, or purification of V5-tagged fusion proteins and complexes. The V5-tag is a 14-amino-acid peptide (GKPIPNPLLGLDST) derived from simian parainfluenza virus V5; its nucleotide sequence is fused to the 5′ or 3′ end of the target gene to generate V5-tagged recombinant proteins.
Applications	IP, Co-IP, Protein purification
Particle Size	200 nm
Concentration	10 mg/mL
Surface Groups	≥ 0.6 mg V5 antibody/mL beads
Magnetic Type	Superparamagnetic
Coupled Antibody	Anti-V5 mouse monoclonal antibody
Isotype	IgG2b
Specificity	V5-Protein, Protein-V5
Elution method	Acid, peptide competitive or SDS-PAGE loading buffer elution. Note: If elute with SDS-PAGE loading buffer, the light (~25kDa) and heavy (~50kDa) chain of V5 antibody will be denatured and release from the beads.
Ligand-binding Capacity	≥ 0.6 mg V5-tagged fusion protein/mL beads
pH	6-8

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