Anti-HIS Magnetic Beads

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Product Infomation

Product Name	Anti-HIS Magnetic Beads
Catalog Number	MGB-IP-023
Size	0.5 mL×1, 0.5 mL×4
Physical Specs	200 nm
Description	Anti-HIS magnetic beads are prepared by covalently coupling high-grade mouse monoclonal anti-His antibody to nanoscale magnetic beads, which specifically bind to His-tagged proteins in plant, animal or microbial lysates, serum, ascites and other samples for IP, Co-IP, or purification of His-tagged fusion proteins and complexes. The His-tag consists of six consecutive histidine residues (HHHHHH); its coding sequence is fused to the 5′ or 3′ end of the target gene to express His-tagged recombinant proteins.
Applications	IP, Co-IP, Protein purification
Particle Size	200 nm
Concentration	10 mg/mL
Surface Groups	≥ 0.6 mg HIS antibody/mL beads
Magnetic Type	Superparamagnetic
Coupled Antibody	Anti-HIS mouse monoclonal antibody
Isotype	IgG1
Specificity	Met-His-Protein, His-Protein, Protein-His
Elution method	Acid, peptide competitive or SDS-PAGE loading buffer elution. Note: If elute with SDS-PAGE loading buffer, the light (~25kDa) and heavy (~50kDa) chain of HIS antibody will be denatured and release from the beads.
Ligand-binding Capacity	≥ 0.6 mg HIS-tagged fusion protein/mL beads
pH	6-8

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