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Product Infomation
Product Name Anti-GFP Magnetic Beads
Catalog Number MGB-IP-025
Size 0.5 mL×1, 0.5 mL×4
Physical Specs 200 nm
Description Anti-GFP magnetic beads are fabricated by covalently coupling premium mouse monoclonal anti-GFP antibody to nanoscale magnetic beads, which specifically bind to GFP-tagged proteins in plant, animal or microbial lysates, serum, ascites and other samples for IP, Co-IP, or purification of GFP-tagged fusion proteins and complexes. Green fluorescent protein (GFP) emits green fluorescence upon UV or blue light excitation. GFP and its enhanced variant EGFP are widely used in studies of gene expression regulation, transgene function, protein localization, trafficking, and high-throughput drug screening. As a live-cell probe, GFP enables real-time visualization of protein expression in living cells via fluorescence microscopy without cell lysis, substrates, or antibodies, offering high stability, sensitivity, and reproducibility. However, its ~25 kDa size may interfere with the function of fused target proteins.
Applications IP, Co-IP, Protein purification
Particle Size 200 nm
Concentration 10 mg/mL
Surface Groups ≥ 0.6 mg GFP antibody/mL beads
Magnetic Type Superparamagnetic
Coupled Antibody Anti-GFP mouse monoclonal antibody
Isotype IgG1
Specificity GFP-Protein, Protein-GFP
Elution method Acid, peptide competitive or SDS-PAGE loading buffer elution. Note: If elute with SDS-PAGE loading buffer, the light (~25kDa) and heavy (~50kDa) chain of GFP antibody will be denatured and release from the beads.
Ligand-binding Capacity ≥ 0.6 mg GFP-tagged fusion protein/mL beads
pH 6-8
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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