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Product Infomation
Product Name 3 μm Time-Resolved Fluorescent Microspheres
Catalog Number FLM-TF-013
Physical Specs 3 μm
Cat NO FLM-TF-013
Description Time-resolved fluorescence immunoassay utilizes rare-earth ions with long fluorescence half-lives as labels. Because these labels have a large Stokes shift (>150 nm) and a fluorescence lifetime that is 5 to 6 orders of magnitude longer than that of background substances, therefore, during measurement, simply delaying the detection time until the background fluorescence has fully decayed before measuring the labeled signal can effectively eliminate interference from various nonspecific fluorescence sources, resulting in very high sensitivity. Time-resolved fluorescent microspheres, as a special type of functional microsphere, can encapsulate tens of thousands of fluorescent molecules within each microsphere, significantly improving the efficiency of fluorescent labeling and effectively enhancing analytical sensitivity; simultaneously, the surface of the fluorescent microspheres is modified with an appropriate density of carboxyl groups or other functional groups for covalent coupling with proteins or antibodies, thereby enhancing the stability of the labeled molecules.
Applications This product demonstrates exceptional application potential and value in in vitro diagnostics and immunochromatographic assays. In clinical settings, time-resolved fluorescent microspheres are widely used in diverse diagnostic panels, including thyroid function tests (TSH, T3, T4), reproductive hormone assays (FSH, LH, HCG), tumor marker detection (AFP, CEA, PSA), infectious disease screening (HBsAg, HBsAb), inflammatory biomarker analysis (SAA, CRP), and cardiovascular disease evaluation (cTnI, NT-proBNP). Within immunochromatography, time-resolved fluorescence immunochromatographic assay (TRFMIA) enables simultaneous detection of multiple serum tumor markers such as Alpha-Fetoprotein (AFP) and Carcinoembryonic Antigen (CEA).
Features The rare earth europium dye is embedded within the microsphere matrix, where it emits light in a concentrated manner, resulting in a more stable fluorescent signal; lanthanide chelates possess a longer fluorescence lifetime and a greater Stokes shift. Therefore, during measurement, extending the measurement time helps mitigate non-specific fluorescence interference and enhances detection sensitivity; A 5% content of rare earth europium ensures high-intensity, high-brightness fluorescence signals; particle size is highly uniform (CV% < 5%), with minimal batch-to-batch variation and excellent reproducibility.
Particle Size 3 μm
Concentration 1% solids content
Surface Groups -COOH
Shell Polystyrene (PS)
Storage Solution DI Water
Excitation Wavelength 365 nm
Emission Wavelength 615 nm
Color Silver-white
Staining Method Internal filling
Homogeneity C.V.＜5%
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