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Product Infomation
Product Name Recombinant Human Laminin, His
Catalog Number OGF-008
Size 10μg;100μg;1mg
Description Laminin (LN), also known as laminin, is a glycoprotein with a unique structure. It has a molecular weight of up to 805 kDa and consists of one heavy α-chain (400 kDa) and two light β-chains (β1 and β2, approximately 200 kDa each); its length is equivalent to the thickness of the basement membrane. It is a non-collagenous glycoprotein that constitutes the extracellular matrix. In the liver, it is primarily synthesized by endothelial cells and lipoencytes, and together with collagen, it forms the components of the basement membrane. Its biological function is to serve as a mediator for cell adhesion to the matrix; it binds to various basement membrane components to regulate cell growth and differentiation. It is a multifunctional molecule composed of multiple structural domains, possessing binding sites for molecules such as type IV collagen, tendinins, heparan sulfate, heparin, galactocerebroside, and gangliosides. It serves as one of the primary components of various basement membranes in the embryonic and adult tissues of various animals. LN is primarily located in the lamina lucida of the basement membrane, adjacent to the basal surface of cells, and is typically absent from the apical and lateral surfaces of normal cells. However, in malignant cells, its distribution is not limited to the basal surface. Tumor cells with high metastatic potential exhibit higher levels of LN on their surfaces. In addition, LN is present in small amounts in the dermal connective tissue of rats and between embryonic cells. To date, the only cells not associated with LN are chondrocytes and the hematopoietic cell system of the bone marrow. The concentration of LN in blood and tissue fluids is extremely low.
Application This product can be used for research on providing structural support and cell adhesion motifs for engineered tissue scaffolds with His-tagged human laminin for biomimetic matrices.
Molecular Weight 17.7 KDa
Species Human
Source E.coli
Appearance White loose or powdery freeze-dried product
Purity > 95% by SDS-PAGE
Endotoxin Level < 1 EU/µg
Redissolution Dissolve with sterile water for injection or triple-distilled water.
Storage Store at -20°C.
Shelf Life 1 year
Note Add an appropriate amount of sterile distilled water to dissolve completely, and dilute to a concentration of 0.1-1 mg/mL. Allow the solution to stand at room temperature for 15-20 minutes to ensure complete dissolution.
The resuspended protein solution can be stored at 2-8°C for approximately 1 week, or aliquoted and stored at 20°C to -80°C for 3-6 months.
For long-term storage, to enhance protein stability, it is recommended to dissolve the protein in 0.1% BSA at a concentration of no less than 10 μg/mL and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Please refer to the literature to determine the optimal working concentration, or determine and optimize the working concentration yourself based on the experimental purpose, specific cells, or animals.
For research use only. Not for use in diagnostics, as a drug, or in humans.
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