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Product Infomation
Product Name Recombinant Human Acidic Fibroblast Growth Factor, Sf9
Catalog Number FGF-009
Size 2µg;10µg;1mg
Description Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. Members of the FGF family possess broad mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This protein acts as a regulator of endothelial cell migration and proliferation and is also a angiogenic factor. In vitro, it acts as a mitogen for various mesodermal and neuroectodermal cells and is therefore believed to be involved in organogenesis. Currently, three selectively spliced variants encoding different subtypes have been identified. Bound growth factors exhibit angiogenic effects in vivo and possess potent mitogenic activity on various cell types in vitro. These two growth factors differ in their tissue distribution and concentration.
Application This product can be used for research on promoting endothelial cell proliferation for vascularization in engineered constructs using Sf9-derived human acidic FGF.
Molecular Weight 15.8 kDa
Species Human
Source Sf9, Baculovirus cells
Appearance Sterile-filtered liquid
Purity ≥ 95% by SDS-PAGE, RP-HPLC
Activity The ED₅₀, calculated based on the dose-dependent proliferation of BAF3 cells expressing FGF receptors (as measured by 3H-thymidine uptake), was < 10 ng/mL, corresponding to an activity of 10⁵ IU/mg.
Formulation The sterile protein solution (1.8 mg/mL) contains 20 mM Tris-HCl (pH 7.9), 100 mM KCl, 0.2 mM DTT, and 20% glycerol.
Storage Store at 4°C for 2-4 weeks. For long-term storage, freeze at -20°C. For long-term storage, we recommend adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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