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Product Infomation
Catalog Number NAT-0017
Product Name Collagen Type I solution from rat tail
Synonyms Collagen
Description Col1a1 (Collagen, type I, α1) or Collagen is a major structural human protein, which assembles in the form of fibrils. It is a very long, thin and the most abundant protein found in the human body. It is a supercoiled right-helix of three left-handed polypeptide chains. These chains are composed of ~1040 amino acids, which are essentially repeats of three amino acids -(Gly-X-Y)n. Gly is glycine and X and Y can be any amino acids, but in humans are usually proline and hydroxyproline, respectively. Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (Collagenase type I) is linked with type I osteogenesis imperfecta. Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.
Morphology & Appearance powder
Source rat tail
Storage 2-8°C
Shipping & Packaging Approx. 100mg/vial (Volume 22 - 25ml/vial, see label for lot-specific protein concentration)
Application Used as a coating material to support adherent cells growth and differentiation.
Usage Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.

Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I Collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the Collagen sample is >95%.
Surface coverage 6-10 μg/cm2
Stability suitable for cell culture
impurities Endotoxin: <0.25 EU/Ml
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