Recombinant Human Collagen

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Recombinant Human Collagen
Product Infomation
Cat.No. NAT-0005
Product Name Recombinant Human Collagen
Synonyms Collagen
Morphology & Appearance powder
Purity ≥95% (SDS-PAGE)
Source expressed in Nicotiana tabacum (tobacco)
Storage 20°C
Reconstitution Soluble in 10 mM HCl at ≤ 3.5 mg/mL. Solutions should be stored at 2-8 °C.
Application This recombinant type I human Collagen is suitable for cell culture applications, including surface coating and the fabrication of scaffolds and 3-Dimensional matrices. This Collagen is prepared from bioengineered plants and is free of human-derived components such as as cytokines, hormones, and growth factors.
Usage Resuspend in 10 mM HCl to the desired concentration. Good solubility expected at a concentration between 0.3% and 10%.
Coating of tissue culture dishes: Use 3 to 10 microgram per 1 cm2 dish area. For each cell type and plate type an optimization may be required. Dilute in 10 mM HCl or PBS. Apply the diluted Collagen to a tissue culture plate and allow to dry overnight in a laminar flow hood with the plate lid open. Aspirate gently the remaining liquid from the coated wells. Wash twice gently with PBS.
To prepare a Collagen gel the fibrillogenesis buffer contains 162 mM sodium phosphate dibasic (Na2HPO4) adjusted to pH 11.2 with 10 N NaOH, filter sterilized. Mix 9 volumes of Collagen at a concentration of 0.3%-1% with 1 volume of fibrillogenesis buffer. Mix well and incubate for 4 to 16 hours at 25°C to 27 °C. Higher concentration of Collagen solution might need optimization. Thiis preparation will not spontaneously form a gel in PBS or cell culture medium.
Preparation of Collagen sponge: concentrate gel to 10 mg/ml - 100 mg/ml by centrifugation, freeze dry. Crosslink by common methods found in the scientific literature such as 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), Glutaraldehyde or thermal dehydration.
Other forms: membranes, sheets, fibers can be prepared by methods commonly used with other types of Collagen.
Description Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub.
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