Dextran (RGD)-based CD-Link Hydrogel Kit
Catalog No: HG001
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| Product Description | The Dextran (RGD)-based CD-Link Hydrogel Kit contains reagents for the preparation of hydrogels with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol/L. The gel composition allows three-dimensional spreading and migration of most cells. The gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) with CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide. The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It allows cells to spread and migrate within the gel if they express the indicated MMPs. Dextran (RGD)-based CD-Link Hydrogel can be dissolved by the addition of dextranase, which allows recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or further cultivation. |
| Application | 3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation. |
| Components | RGD-Dextran, lyophilized: 2 x 870 µL* CD-Link, lyophilized: 200 µL*, 20 mmol/L* Reconstitution Buffer: 2 x 900 µL Water: 600 µL |
| Storage/Stability | Store at -20 °C. |
| Note | Allows formation of 2.4 mL Dextran(RGD)-based CD-Link Hydrogel. |
! For Research/Industry Use Only!